SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Oblessuc,Paula Rodrigues; Campos,Tatiana de; Cardoso,Juliana Morini Kupper; Sforça,Danilo Augusto; Baroni,Renata Moro; Souza,Anete Pereira de; Benchimol,Luciana Lasry.

Capacidade combinatória, divergência genética entre linhagens de milho e correlação com heterose

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000600013

Bragantia

Paterniani,Maria Elisa Ayres Guidetti Zagatto; Guimarães,Paula de Souza; Lüders,Reginaldo Roberto; Gallo,Paulo Boller; Souza,Anete Pereira de; Laborda,Prianda Rios; Oliveira,Karine Miranda.

Objetivou-se avaliar o desempenho de híbridos simples de milho (Zea mays L.), obtidos de cruzamentos dialélicos entre linhagens divergentes, estimar a capacidade de combinação das linhagens e verificar se a divergência genética entre as linhagens, obtida por marcadores moleculares, é correlacionada com a heterose dos híbridos simples no campo. Trinta e seis híbridos resultantes de um dialelo parcial foram avaliados em Campinas e em Mococa e as 12 linhagens parentais somente em Campinas, a fim de se calcular a heterose dos híbridos. O delineamento experimental empregado foi o de blocos ao acaso, com três repetições e 2 testemunhas comerciais. Avaliaram-se os caracteres: altura da planta (AP), altura da espiga (AE) e massa de grãos (MG). Análises de...

Comparison of similarity coefficients used for cluster analysis with dominant markers in maize (Zea mays L)

Palavras-chave: Zea mays; Dialelo parcial; Produtividade; AFLP; SSR.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052008000300012

Meyer,Andréia da Silva; Garcia,Antonio Augusto Franco; Souza,Anete Pereira de; Souza Jr.,Cláudio Lopes de.

The objective of this study was to evaluate whether different similarity coefficients used with dominant markers can influence the results of cluster analysis, using eighteen inbred lines of maize from two different populations, BR-105 and BR-106. These were analyzed by AFLP and RAPD markers and eight similarity coefficients were calculated: Jaccard, Sorensen-Dice, Anderberg, Ochiai, Simple-matching, Rogers and Tanimoto, Ochiai II and Russel and Rao. The similarity matrices obtained were compared by the Spearman correlation, cluster analysis with dendrograms (UPGMA, WPGMA, Single Linkage, Complete Linkage and Neighbour-Joining methods), the consensus fork index between all pairs of dendrograms, groups obtained through the Tocher optimization procedure and...

Correlação da heterose de híbridos de milho com divergência genética entre linhagens

Palavras-chave: Genetic divergence; Heterotic groups; AFLP; RAPD; Multivariate analysis.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100014

Guimarães,Paula de Souza; Paterniani,Maria Elisa Ayres Guidetti Zagatto; Lüders,Reginaldo Roberto; Souza,Anete Pereira de; Laborda,Prianda Rios; Oliveira,Karine Miranda.

O objetivo deste trabalho foi avaliar a produtividade de híbridos simples de milho obtidos de cruzamentos dialélicos entre linhagens divergentes, estimar a capacidade de combinação das linhagens e confirmar se a divergência genética entre as linhagens, obtida por marcadores moleculares, é correlacionada com a heterose dos híbridos simples no campo. Trinta e seis híbridos resultantes do dialelo parcial e as 12 linhagens parentais foram avaliadas em Campinas em blocos ao acaso, com três repetições e duas testemunhas. A capacidade de combinação das linhagens foi estimada de acordo com o modelo 4 de Griffing. Estimaram-se a correlação matricial, mediante a estatística de Mantel, entre heterose, produtividade e capacidade específica de combinação com...

Genetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers

Palavras-chave: Zea mays; Marcadores moleculares; Dialelo parcial; Capacidade de combinação; AFLP; SSR.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000600007

Scientia Agricola

Sousa,Adna Cristina Barbosa de; Godoy,Rodolfo; Sforça,Danilo Augusto; Campos,Tatiana de; Zucchi,Maria Imaculada; Jank,Liana; Souza,Anete Pereira de.

The pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49) and the D values from 0.23 to 0.91 (average 0.58). The averages of observed and expected heterozygosity were 0.25 and 0.47,...

Genetic diversity in cultivated carioca common beans based on molecular marker analysis

Palavras-chave: Legumes; Transferability; Microsatellite; Germplasm.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400007

Perseguini,Juliana Morini Küpper Cardoso; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Colombo,Carlos Augusto; Carbonell,Sérgio Augusto Moraes; Mondego,Jorge Mauricio Costa; Gazaffi,Rodrigo; Garcia,Antonio Augusto Franco; Campos,Tatiana de; Souza,Anete Pereira de; Rubiano,Luciana Benchimol.

A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were...

Microsatellite-assisted backcross selection in maize

Palavras-chave: AFLPs; Genetic structure; Genetic variability; Phaseolus vulgaris L.; SSRs.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017

Benchimol,Luciana Lasry; Souza Jr.,Cláudio Lopes de; Souza,Anete Pereira de.

A microsatellite marker (SSR) was chosen to simulate a target allele and three criteria (02, 04 and 06 markers per chromosome) were tested to evaluate the most efficient parameters for performing marker-assisted backcross (MAB) selection. We used 53 polymorphic SSRs to genotype 186 BC1 maize (Zea mays L.) plants produced by crossing the inbred maize lines L-08-05 (donor parent) and L-14-4B (recurrent parent). The second backcross (BC2) generation was produced with 180 plants and screened with markers which were not recovered from the first backcross (BC1) generation. A total of 480 plants were evaluated in the third backcross (BC3) generation from which 48 plants were selected for parental genotype recovery. Recurrent genotype recovery averages in three...

Microsatellites for genetic studies and breeding programs in common bean

Palavras-chave: Maize; Marker-assisted backcrossing (MAB); Marker-assisted selection; Microsatellites.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500022

Campos,Tatiana de; Benchimol,Luciana Lasry; Carbonell,Sérgio Augusto Moraes; Chioratto,Alisson Fernando; Formighieri,Eduardo Fernandes; Souza,Anete Pereira de.

Twenty microsatelitte loci were identified and characterized in common bean. Microsatellites were tested in 14 genotypes. The allele number ranged from 1 to 3, and the polymorphism information content (PIC) was between 0.14 and 0.65. These polymorphic markers are available to be used for breeding programs.

New microsatellite markers developed from an enriched microsatellite common bean library

Palavras-chave: Phaseolus vulgaris; SSR; Molecular markers.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000400018

Cardoso,Juliana Morini Kupper; Oblessuc,Paula Rodrigues; Campos,Tatiana de; Sforça,Danilo Augusto; Carbonell,Sérgio Augusto Moraes; Chioratto,Alisson Fernando; Formighieri,Eduardo Fernandes; Souza,Anete Pereira de; Benchimol,Luciana Lasry.

The objective of this work was to develop new microsatellite markers in common bean. Ninety nine new microsatelitte loci were developed from a microsatellite enriched library for (CT)8 and (GT)8 motifs, from CAL-143 line. The majority of microsatellite sequences (51%) was related to cellular metabolism. The remaining sequences were associated to transcription functions. Only 17.2% of the sequences presented some level of similarity with other plant species genes.

PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze

Palavras-chave: Phaseolus vulgaris; Molecular markers; Plant breeding; QTL; SSR.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700019

Schlögl,Paulo Sérgio; Souza,Anete Pereira de; Nodari,Rubens Onofre.

The Araucaria angustifolia (Bert.) O. Kuntze, also named the "paraná pine" (pinheiro-do-Paraná in Portuguese), is a native conifer species naturally occurring in the Brazilian Tropical Atlantic Forest which in Brazil is mostly limited to the southern Brazilian states of Paraná, Santa Catarina and Rio Grande do Sul. Chloroplast DNA markers (cpDNA) are useful in populational genetic studies because of their low substitution rate and the uniparental transmission. The conservation of cpDNA genes between species has allowed the design of consensus chloroplast primers that have had a great impact on population genetics and phylogenetic studies. In this study we used the polymerase chain reaction technique combined with restriction enzyme fragment length...

Potato cultivar identification using molecular markers

Palavras-chave: Haplotypes; Conifer species; Brazilian pine; Genetic diversity.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300020

Rosa,Patricia Marchezi; Campos,Tatiana de; Sousa,Adna Cristina Barbosa de; Sforça,Danilo Augusto; Torres,Gisele Abigail Montan; Souza,Anete Pereira de.

The objective of this work was to evaluate a set of microsatellite markers for varietal identification and characterization of the most widespread potato cultivars in Brazil. The DNA from 14 potato cultivars was genotyped using microsatellite markers and the alleles were scored in silver-stained polyacrylamide gel. Twenty-four microsatellite markers were evaluated, and only one locus was monomorphic. Based on band patterns, a set of two microsatellites that were able to identify and differentiate all examined cultivars was obtained.

Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci

Palavras-chave: Solanum; AFLP; Genetic certification; Varietal protection; Variety identification; RAPD.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000100015

Pinto,Luciana Rossini; Vieira,Maria Lucia Carneiro; Souza Jr.,Claudio Lopes de; Souza,Anete Pereira de.

A modified reciprocal recurrent selection (RRS) method, which employed one cycle of high-intensity selection, was applied to two tropical maize (Zea mays L.) populations, BR-105 and BR-106, originating the improved synthetics IG-3 and IG-4, respectively. In the present study the effects of this kind of selection on the genetic structure of these populations and their synthetics were investigated at 30 microsatellite (SSR) loci. A total of 125 alleles were revealed. A reduction in the number of alleles was observed after selection, as well as changes in allele frequencies. In nearly 13% (BR-105) and 7% (BR-106) of the loci evaluated, the changes in allele frequencies were not explained, exclusively due to the effects of genetic drift. The effective...

Using genetic diversity information to establish core collections of Stylosanthes capitata and Stylosanthes macrocephala

Palavras-chave: Population genetics; Plant breeding; Molecular markers.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300022

Santos-Garcia,Melissa Oliveira; Toledo-Silva,Guilherme de; Sassaki,Rodrigo Possidonio; Ferreira,Thais Helena; Resende,Rosângela Maria Simeão; Chiari,Lucimara; Karia,Cláudio Takao; Carvalho,Marcelo Ayres; Faleiro,Fábio Gelape; Zucchi,Maria Imaculada; Souza,Anete Pereira de.

Stylosanthes species are important forage legumes in tropical and subtropical areas. S. macrocephala and S. capitata germplasm collections that consist of 134 and 192 accessions, respectively, are maintained at the Brazilian Agricultural Research Corporation Cerrados (Embrapa-Cerrados). Polymorphic microsatellite markers were used to assess genetic diversity and population structure with the aim to assemble a core collection. The mean values of H O and H E for S. macrocephala were 0.08 and 0.36, respectively, whereas the means for S. capitata were 0.48 and 0.50, respectively. Roger's genetic distance varied from 0 to 0.83 for S. macrocephala and from 0 to 0.85 for S. capitata. Analysis with STRUCTURE software distinguished five groups among the S....